phpMs Demo Suite

Protein Digestion

This tool allows you to upload a FASTA file and to generate a list of peptides as would be produced by the chosen enzyme. You may filter the peptides to those within a certain mass. The output will be a .csv file.

The NME option sets whether NME should be performed. When enabled any methionine at the n-terminus of a protein will be removed. Both the excised and non-excised peptide will be returned after digestion. Note, protein sequence which do not contain a methionine at the n-terminus will be unaffected.

Da ± ppm